Fermentas TopVision™ Agarose R0491

R0491 100 g  
R0492 500 g
Features

Optimal concentration between 0.4-5% in all typical buffer systems.
GQ (Genetic Quality) certified, which ensures that nucleic acids recovered from preparative gels can be used for downstream applications (enzymatic reactions etc.).
Low DNA/RNA binding.
Excellent gel transparency.
DNase and RNase free.
Applications

Analytical electrophoresis of nucleic acids.
Preparative electrophoresis.
Blotting assays.
Characteristics
Electroendosmosis EEO – 0.05-0.13
Gel strength (1% gel) – >1200 g/cm2
Gel strength (1.5% gel) – >2500 g/cm2
Gelling temperature – 36±1.5°C
Melting temperature – 88±1.5°C
Moisture – <7%

Quality Control
The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.

Fermentas 6X DNA Loading Dye R0611

Features

Two-color tracking of DNA migration during electrophoresis.
No DNA masking during gel exposure to UV light.
EDTA binds divalent metal ions and inhibits metal dependent nucleases.
Applications

Preparation of DNA ladders, markers and samples for loading on agarose or polyacrylamide gels.
Description
6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solution binds divalent metal ions and inhibits metal-dependent nucleases.

6X DNA Loading Dye is used for conventional DNA electrophoresis (see recommendations for use).

Quality Control
Tested for DNA sample preparation prior to agarose gel electrophoresis. The absence of deoxyribonucleases confirmed by appropriate tests.

Composition
10 mM Tris-HCl (pH 7.6),
0.03% bromophenol blue,
0.03% xylene cyanol FF,
60% glycerol,
60 mM EDTA

Storage
Store at room temperature or at 4°C up to 12 months.
For longer periods, store at -20°C.

Fermentas GeneRuler™ 100 bp DNA Ladder, 100-1000 bp

SM0241 50 µg (0.5 µg/µl) 1.00 ml SM0241
SM0242 250 (5x50) µg (0.5 µg/µl) 2 x 1.00 ml SM0242
Features

Ideal for both DNA sizing and approximate quantification.
Sharp bands.
Bright reference bands (given in red).
Supplied with loading dye for sample DNA.
Description
GeneRuler™ 100 bp DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler™ 100 bp DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase, see protocol.

The ladder is supplied with 6X DNA Loading Dye.

Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.

6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.

Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The DNA concentration is determined spectrophotometrically.
The absence of nucleases is confirmed by a direct nuclease activity assay.

Storage
Store at -20°C.

Fermentas 10X TBE Buffer (Tris-borate-EDTA) Cat. B52

Applications

Electrophoresis of nucleic acids in agarose and polyacrylamide gels.
Used both as a running buffer and as a gel preparation buffer.
Filtered through a 0.22 µm membrane.
Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 b(p).
1X Composition
89 mM Tris,
89 mM boric acid,
2 mM EDTA,
pH of 10X TBE: 8.3

Usage Recommendations
Use fresh 1X TBE both for the gel and for the electrophoresis run.
To prepare 1X TBE buffer, add 100 ml of 10X TBE buffer to 900 ml of deionized water and mix well.

Quality Control
The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.

Note
Double-stranded linear nucleic acid molecules migrate about 10% slower in TBE buffer than in TAE buffer.

Storage
There are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a precipitate may form, which is easily dissolved by gentle heating.

Fermentas TAE 50X Buffer ( Tris-acetate-EDTA) Cat. B49

Electrophoresis of nucleic acids in agarose and polyacrylamide gels.
Used both as a running buffer and as a gel preparation buffer.
Filtered through a 0.22 µ m membrane.
Recommended for resolution of RNA and DNA fragments larger than 1500 b( p) , for genomic DNA and for large supercoiled DNA.
1X Composition
40 mM Tris,
20 mM acetic acid,
1 mM EDTA,
pH of 50X TAE: 8.4

Usage Recommendations
Use fresh 1X TAE both for the gel and for the electrophoresis run.
To prepare 1X TAE buffer add 20 ml of 50X TAE buffer to 980 ml of deionized water and mix well.

Quality Control
The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.