Fermentas GeneRuler™ 100 bp DNA Ladder, 100-1000 bp

SM0241 50 µg (0.5 µg/µl) 1.00 ml SM0241
SM0242 250 (5x50) µg (0.5 µg/µl) 2 x 1.00 ml SM0242
Features

Ideal for both DNA sizing and approximate quantification.
Sharp bands.
Bright reference bands (given in red).
Supplied with loading dye for sample DNA.
Description
GeneRuler™ 100 bp DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler™ 100 bp DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase, see protocol.

The ladder is supplied with 6X DNA Loading Dye.

Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.

6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.

Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The DNA concentration is determined spectrophotometrically.
The absence of nucleases is confirmed by a direct nuclease activity assay.

Storage
Store at -20°C.