Fermentas 10X TBE Buffer (Tris-borate-EDTA) Cat. B52

Applications

Electrophoresis of nucleic acids in agarose and polyacrylamide gels.
Used both as a running buffer and as a gel preparation buffer.
Filtered through a 0.22 µm membrane.
Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 b(p).
1X Composition
89 mM Tris,
89 mM boric acid,
2 mM EDTA,
pH of 10X TBE: 8.3

Usage Recommendations
Use fresh 1X TBE both for the gel and for the electrophoresis run.
To prepare 1X TBE buffer, add 100 ml of 10X TBE buffer to 900 ml of deionized water and mix well.

Quality Control
The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.

Note
Double-stranded linear nucleic acid molecules migrate about 10% slower in TBE buffer than in TAE buffer.

Storage
There are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a precipitate may form, which is easily dissolved by gentle heating.